| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Sinorhizobium fredii | ATGCACGTTGTCGGCACA | TCTCGGTGTTGGCGAGTTC | 60.59 | 60.01 | 187 | 92 |
100.00%
|
100.00%
|
| Primer 22 | Sinorhizobium fredii | ATGCACGTTGTCGGCACA | TGTTCGGTTGGCCTGTCTG | 60.59 | 60.23 | 232 | 92 |
100.00%
|
100.00%
|
| Primer 23 | Sinorhizobium fredii | TCGGTTCGCGTGTTCGAA | TTTCGTGCGACTCGTGCA | 59.97 | 60.28 | 240 | 92 |
100.00%
|
100.00%
|
| Primer 24 | Sinorhizobium fredii | CGTAGAGTTCGTCGGTGCT | ATCGCCTGTTGGTCGTGT | 59.50 | 59.26 | 225 | 92 |
100.00%
|
100.00%
|
| Primer 25 | Sinorhizobium fredii | GCACATTGGGCAAGCTCAC | TGCAATGGACGGCATCGA | 60.08 | 59.73 | 153 | 91 |
100.00%
|
100.00%
|
| Primer 26 | Sinorhizobium fredii | CGGTTTTGGCGACATCACG | TGCAATGGACGGCATCGA | 60.15 | 59.73 | 179 | 91 |
100.00%
|
100.00%
|
| Primer 27 | Sinorhizobium fredii | CCGGTTTTGGCGACATCAC | TGCAATGGACGGCATCGA | 59.79 | 59.73 | 180 | 91 |
100.00%
|
100.00%
|
| Primer 28 | Sinorhizobium fredii | ACCGGTTTTGGCGACATCA | TGCAATGGACGGCATCGA | 60.23 | 59.73 | 181 | 91 |
100.00%
|
100.00%
|
| Primer 29 | Sinorhizobium fredii | GCACATTGGGCAAGCTCA | TGCAATGGACGGCATCGA | 58.63 | 59.73 | 153 | 91 |
100.00%
|
100.00%
|
| Primer 30 | Sinorhizobium fredii | ACAATTGGCTGGAGGCGA | AGCAGCTTTTCCAGGCGT | 59.24 | 59.89 | 277 | 91 |
100.00%
|
100.00%
|